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GLI1 Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GLI1 Antibody (N-Term) AP21920a
    Anti-GLI1 Antibody (N-Term) at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08151
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 117904 Da
Additional Information
Gene ID 2735
Other Names Zinc finger protein GLI1, Glioma-associated oncogene, Oncogene GLI, GLI1, GLI
Target/Specificity This GLI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 196-230 amino acids from human GLI1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGLI1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GLI1
Synonyms GLI
Function Acts as a transcriptional activator (PubMed:10806483, PubMed:19706761, PubMed:19878745, PubMed:24076122, PubMed:24217340, PubMed:24311597). Binds to the DNA consensus sequence 5'-GACCACCCA-3' (PubMed:2105456, PubMed:24217340, PubMed:8378770). Regulates the transcription of specific genes during normal development (PubMed:19706761). Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling (PubMed:19706761, PubMed:28973407). Plays a role in cell proliferation and differentiation via its role in SHH signaling (PubMed:11238441, PubMed:28973407).
Cellular Location Cytoplasm. Nucleus. Note=Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441)
Tissue Location Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761)
Research Areas
Citations (0)
citation

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Background

Acts as a transcriptional activator. May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

References

Kinzler K.W.,et al.Nature 332:371-374(1988).
Yoon J.W.,et al.Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
Lo H.W.,et al.Cancer Res. 69:6790-6798(2009).
Scherer S.E.,et al.Nature 440:346-351(2006).
Murone M.,et al.Nat. Cell Biol. 2:310-312(2000).

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$ 182.50
$ 70.00
Cat# AP21920a
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