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SKI Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SKI Antibody (N-Term) AP21908a
    All lanes : Anti-SKI Antibody (N-Term) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: A549 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 80 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P12755
Other Accession Q9TUG2
Reactivity Human
Predicted Horse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 80005 Da
Additional Information
Gene ID 6497
Other Names Ski oncogene, Proto-oncogene c-Ski, SKI
Target/Specificity This SKI antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-54 amino acids from human SKI.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSKI Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SKI
Function May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.
Cellular Location Nucleus.
Research Areas
Citations (0)
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Background

May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling.

References

Nomura N.,et al.Nucleic Acids Res. 17:5489-5500(1989).
Gregory S.G.,et al.Nature 441:315-321(2006).
Harada J.,et al.J. Biol. Chem. 278:38998-39005(2003).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
Takahata M.,et al.J. Biol. Chem. 284:3334-3344(2009).

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$ 365.00
$ 140.00
Cat# AP21908a
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