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PYGL Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PYGL Antibody (C-Term) AP21847b
    All lanes : Anti-PYGL Antibody (C-Term) at 1:2000 dilution Lane 1: human liver lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - PYGL Antibody (C-Term) AP21847b
    AP21847b staining PYGL in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P06737
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 97149 Da
Additional Information
Gene ID 5836
Other Names Glycogen phosphorylase, liver form, PYGL
Target/Specificity This PYGL antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 809-843 amino acids from human PYGL.
Dilution WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPYGL Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PYGL (HGNC:9725)
Function Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
Cellular Location Cytoplasm, cytosol.
Research Areas
Citations (0)
citation

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Background

Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

References

Newgard C.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 83:8132-8136(1986).
Carty M.D.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Chang S.,et al.Hum. Mol. Genet. 7:865-870(1998).
Burwinkel B.,et al.Am. J. Hum. Genet. 62:785-791(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 365.00
$ 140.00
Cat# AP21847b
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