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H3f3b Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P84244 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15328 Da |
| Gene ID | 15078;15081 |
|---|---|
| Other Names | Histone H33, H3f3a, H33a |
| Target/Specificity | This H3f3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 31-65 amino acids from human H3f3b. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | H3f3b Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | H3-3a {ECO:0000250|UniProtKB:P84243} |
|---|---|
| Function | Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Cellular Location | Nucleus. Chromosome. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
References
Hraba-Renevey S.,et al.Nucleic Acids Res. 17:2449-2461(1989).
Bramlage B.,et al.Differentiation 62:13-20(1997).
Lopez-Alanon D.M.,et al.DNA Cell Biol. 16:639-644(1997).
Carninci P.,et al.Science 309:1559-1563(2005).
Mancini P.,et al.J. Mol. Evol. 59:458-463(2004).
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