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GLS Antibody (C-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O94925 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 73461 Da |
| Gene ID | 2744 |
|---|---|
| Other Names | Glutaminase kidney isoform, mitochondrial, GLS, K-glutaminase, L-glutamine amidohydrolase, GLS, GLS1, KIAA0838 |
| Target/Specificity | This GLS antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 555-587 amino acids from human GLS. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GLS Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GLS |
|---|---|
| Synonyms | GLS1, KIAA0838 |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188). |
| Cellular Location | [Isoform 1]: Mitochondrion {ECO:0000250|UniProtKB:P13264}. Cytoplasm, cytosol. Note=The 74-kDa cytosolic precursor is translocated into the mitochondria and processed via a 72-kDa intermediate to yield the mature 68- and 65-kDa subunits {ECO:0000250|UniProtKB:P13264} [Glutaminase kidney isoform, mitochondrial 68 kDa chain]: Mitochondrion matrix {ECO:0000250|UniProtKB:P13264} Note=Produced by the proteolytic processing of the 74-kDa cytosolic precursor. {ECO:0000250|UniProtKB:P13264} |
| Tissue Location | Isoform 1 and isoform 3 are detected in brain cortex. Isoform 3 is highly expressed in astrocytoma, ganglioglioma and ependymoma. Isoform 1 is highly expressed in brain and kidney, but not detected in liver. Isoform 3 is highly expressed in heart and pancreas, detected at lower levels in placenta, lung, pancreas and kidney, but is not detected in liver. Isoform 2 is expressed in cardiac and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity.
References
Elgadi K.M.,et al.Physiol. Genomics 1:51-62(1999).
Nagase T.,et al.DNA Res. 5:355-364(1998).
Chavez R.A.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
Holcomb T.,et al.Brain Res. Mol. Brain Res. 76:56-63(2000).
Turner A.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
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