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Zebrafish wnt8a Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Zebrafish wnt8a Antibody (Center) AP21770c
    All lanes : Anti-Zebrafish wnt8a Antibody (Center) at 1:1000-1:2000 dilution Lane 1: zebrafish muscle lysate Lane 2: ZF4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
  • CITATIONS: 1
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P51028
Reactivity Zebrafish
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 40289 Da
Antigen Region 135-170 aa
Additional Information
Gene ID 30122
Other Names Protein Wnt-8a, wnt8a, wnt-8, wnt8
Target/Specificity This Zebrafish wnt8a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 135-170 amino acids from the Central region of Zebrafish wnt8a.
Dilution WB~~1:1000-1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZebrafish wnt8a Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name wnt8a
Synonyms wnt-8, wnt8
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Required for mesoderm and neural ectoderm patterning during gastrulation (PubMed:11703928). Involved in axis formation during embryonic development, via activation of canonical Wnt/CTNNB1 signaling (PubMed:11703928, PubMed:25371059). May be involved in the specification of the spatial patterns of expression of Gsc and other regulatory genes leading to the establishment of the embryonic axis (PubMed:7600994).
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000250|UniProtKB:Q9H1J5}. Secreted {ECO:0000250|UniProtKB:Q9H1J5}
Tissue Location Expressed in the margin of the pregastrula embryo destined to be the future mesoderm.
Research Areas
Citations ( 0 )

Background

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Is likely to signal over only few cell diameters. May be involved in the specification of the spatial patterns of expression of Gsc and other regulatory genes leading to the establishment of the embryonic axis.

References

Kelly G.M.,et al.Development 121:1787-1799(1995).
Lekven A.C.,et al.Dev. Cell 1:103-114(2001).

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$ 365.00
$ 140.00
Cat# AP21770c
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