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HIST3H2A Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HIST3H2A Antibody (N-term) AP21623a
    All lanes : Anti-HIST3H2A Antibody (N-term) at 1:2000 dilution Lane 1: human heart lysate Lane 2: Hela whole cell lysate Lane 3: HT-1080 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: mouse eyeball lysate Lane 6: Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7L7L0
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 14121 Da
Additional Information
Gene ID 92815
Other Names Histone H2A type 3, HIST3H2A
Target/Specificity This HIST3H2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-67 amino acids from the N-terminal region of human HIST3H2A.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST3H2A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name H2AC25 (HGNC:20507)
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Research Areas
Citations (0)
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Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

References

Marzluff W.F.,et al.Genomics 80:487-498(2002).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Aihara H.,et al.Genes Dev. 18:877-888(2004).

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$ 365.00
$ 140.00
Cat# AP21623a
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