GCKR Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q14397 |
Reactivity | Mouse, Rat |
Host | Rabbit |
Clonality | polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 68685 Da |
Gene ID | 2646 |
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Other Names | Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR |
Target/Specificity | This GCKR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 87-120 amino acids from human GCKR. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | GCKR Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | GCKR {ECO:0000303|PubMed:8589523, ECO:0000312|HGNC:HGNC:4196} |
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Function | Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961). |
Cellular Location | Cytoplasm. Nucleus. Mitochondrion {ECO:0000250|UniProtKB:Q07071}. Note=Under low glucose concentrations, GCKR associates with GCK and the inactive complex is recruited to the hepatocyte nucleus. |
Tissue Location | Found in liver and pancreas. Not detected in muscle, brain, heart, thymus, intestine, uterus, adipose tissue, kidney, adrenal, lung or spleen. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Inhibits glucokinase (GCK) by forming an inactive complex with this enzyme. The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6- phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity.
References
Warner J.P.,et al.Mamm. Genome 6:532-536(1995).
Hayward B.E.,et al.Genomics 49:137-142(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).
de la Iglesia N.,et al.FEBS Lett. 456:332-338(1999).
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