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PDHB Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PDHB Antibody (C-term) AP21567b
    All lanes : Anti-PDHB Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: A375 whole cell lysates Lane 3: HepG2 whole cell lysates Lane 4: NCI-H1299 whole cell lysates Lane 5: mouse heart lysates Lane 6: rat heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P11177
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 39233 Da
Additional Information
Gene ID 5162
Other Names Pyruvate dehydrogenase E1 component subunit beta, mitochondrial, PDHE1-B, PDHB, PHE1B
Target/Specificity This PDHB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 324-356 amino acids from the C-terminal region of human PDHB.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDHB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDHB
Synonyms PHE1B
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)
citation

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Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

References

Ho L.,et al.Gene 86:297-302(1990).
Chun K.,et al.Eur. J. Biochem. 194:587-592(1990).
Huh T.L.,et al.J. Biol. Chem. 265:13320-13326(1990).
Koike K.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5594-5597(1990).
Koike K.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:41-45(1988).

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$ 140.00
Cat# AP21567b
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