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VDAC2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VDAC2 Antibody (N-term) AP21559a
    All lanes : Anti-VDAC2 Antibody (N-term) at 1:4000 dilution Lane 1: NCI-H1299 whole cell lysate Lane 2: HCT116 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: Mouse heart tissue lysate Lane 5: Rat heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - VDAC2 Antibody (N-term) AP21559a
    All lanes : Anti-VDAC2 Antibody (N-term) at 1:2000 dilution Lane 1: HCT116 whole cell lysate Lane 2: NCI-H1299 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: Mouse brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - VDAC2 Antibody (N-term) AP21559a
    Immunohistochemical analysis of AP21559a on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • IHC-P - VDAC2 Antibody (N-term) AP21559a
    Immunohistochemical analysis of AP21559a on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P45880
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 31567 Da
Additional Information
Gene ID 7417
Other Names Voltage-dependent anion-selective channel protein 2, VDAC-2, hVDAC2, Outer mitochondrial membrane protein porin 2, VDAC2
Target/Specificity This VDAC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 51-85 amino acids from the N-terminal region of human VDAC2.
Dilution WB~~1:2000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVDAC2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VDAC2
Function Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules (PubMed:38065946). The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV (By similarity). The open state has a weak anion selectivity whereas the closed state is cation-selective (By similarity). Binds various lipids, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol (PubMed:31015432). Binding of ceramide promotes the mitochondrial outer membrane permeabilization (MOMP) apoptotic pathway (PubMed:31015432).
Cellular Location Mitochondrion outer membrane. Membrane. Note=May localize to non-mitochondrial membranes.
Tissue Location Expressed in erythrocytes (at protein level) (PubMed:27641616). Expressed in all tissues examined (PubMed:8420959)
Research Areas
Citations (0)
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Background

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation- selective.

References

Ha H.,et al.J. Biol. Chem. 268:12143-12149(1993).
Blachly-Dyson E.,et al.J. Biol. Chem. 268:1835-1841(1993).
Decker W.K.,et al.Mamm. Genome 10:1041-1042(1999).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 429:375-381(2004).

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$ 365.00
$ 140.00
Cat# AP21559a
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