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Mouse Dnmt3b Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Mouse Dnmt3b Antibody (Center) AP21512c
    All lanes : Anti-Dnmt3b Antibody (Center) at 1:2000 dilution Lane 1: F9 whole cell lysates Lane 2: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O88509
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 97228 Da
Additional Information
Gene ID 13436
Other Names DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase MmuIIIB, DNA MTase MmuIIIB, MMmuIIIB, Dnmt3b
Target/Specificity This Mouse Dnmt3b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 356-390 amino acids from the Central region of Mouse Dnmt3b.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Dnmt3b Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Dnmt3b
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Functions as a transcriptional corepressor by associating with ZHX1 (By similarity). Required for DUX4 silencing in somatic cells (By similarity).
Cellular Location Nucleus. Note=Accumulates in the major satellite repeats at pericentric heterochromatin
Research Areas
Citations (0)
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Background

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Function as transcriptional corepressor by associating with ZHX1 (By similarity).

References

Okano M.,et al.Nat. Genet. 19:219-220(1998).
Yin B.,et al.Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21:431-438(1999).
Okano M.,et al.Cell 99:247-257(1999).
Gowher H.,et al.J. Biol. Chem. 277:20409-20414(2002).
Christophorou M.A.,et al.Nature 507:104-108(2014).

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$ 365.00
$ 140.00
Cat# AP21512c
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