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AKR7L Antibody (N-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - AKR7L Antibody (N-Term) AP21497a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling AKR7L with AP21497a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
    detail
  • WB - AKR7L Antibody (N-Term) AP21497a
    Anti-AKR7L Antibody (N-Term)at 1:2000 dilution + HepG2 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q8NHP1
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 36970 Da
Antigen Region 32-64 aa
Additional Information
Gene ID 246181
Other Names Aflatoxin B1 aldehyde reductase member 4, 1---, AFB1 aldehyde reductase 3, AFB1-AR 3, Aldoketoreductase 7-like, AKR7L, AFAR3, AKR7A4
Target/Specificity This AKR7L antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-64 amino acids from human AKR7L.
Dilution IF~~1:25
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKR7L Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKR7L
Synonyms AFAR3 {ECO:0000303|PubMed:12879023}, AKR
Function Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).
Tissue Location Mainly expressed in uterus.
Research Areas
Citations (0)
citation

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Background

Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen (By similarity).

References

Gregory S.G.,et al.Nature 441:315-321(2006).
Praml C.,et al.Oncogene 22:4765-4773(2003).

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$ 365.00
$ 140.00
Cat# AP21497a
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