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RASSF2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RASSF2 Antibody (Center) AP21484c
    All lanes : Anti-RASSF2 Antibody (Center) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: Raji whole cell lysates Lane 4: human placenta lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - RASSF2 Antibody (Center) AP21484c
    All lanes : Anti-RASSF2 Antibody (Center) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: human brain lysates Lane 4: mouse brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P50749
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 37790 Da
Additional Information
Gene ID 9770
Other Names Ras association domain-containing protein 2, RASSF2, KIAA0168
Target/Specificity This RASSF2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 123-156 amino acids from the Central region of human RASSF2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRASSF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RASSF2
Synonyms CENP-34 {ECO:0000303|PubMed:20813266}, K
Function Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.
Cellular Location Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Translocates to the cytoplasm in the presence of STK3/MST2 and STK4/MST1
Tissue Location Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.
Research Areas
Citations (0)
citation

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Background

Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.

References

Burbee D.G.,et al.Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
Nagase T.,et al.DNA Res. 3:17-24(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Suzuki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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$ 182.50
$ 70.00
Cat# AP21484c
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