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KIF22 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - KIF22 Antibody (Center) AP21475c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF-7 (human breast cancer cell line) cells labeling KIF22 with AP21475c at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on MCF-7 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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  • WB - KIF22 Antibody (Center) AP21475c
    All lanes : Anti-KIF22 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - KIF22 Antibody (Center) AP21475c
    All lanes : Anti-KIF22 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: Hela whole cell lysates Lane 3: SH-SY5Y whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: HL-60 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P - KIF22 Antibody (Center) AP21475c
    AP21475c staining KIF22 in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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  • IHC-P - KIF22 Antibody (Center) AP21475c
    AP21475c staining KIF22 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q14807
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 73262 Da
Additional Information
Gene ID 3835
Other Names Kinesin-like protein KIF22, Kinesin-like DNA-binding protein, Kinesin-like protein 4, KIF22, KID, KNSL4
Target/Specificity This KIF22 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 423-456 amino acids from the Central region of human KIF22.
Dilution IF~~1:25
WB~~1:2000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKIF22 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KIF22
Synonyms KID, KNSL4
Function Kinesin family member that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA (By similarity). Plays a role in congression of laterally attached chromosomes in NDC80-depleted cells (PubMed:25743205).
Cellular Location Nucleus. Cytoplasm, cytoskeleton
Tissue Location Expressed in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes
Research Areas
Citations (0)
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Background

Kinesin family that is involved in spindle formation and the movements of chromosomes during mitosis and meiosis. Binds to microtubules and to DNA.

References

Tokai N.,et al.EMBO J. 15:457-467(1996).
Song J.,et al.Genomics 52:374-377(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP21475c
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