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WNT2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - WNT2 Antibody (Center) AP21468c
    All lanes : Anti-WNT2 Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: human placenta lysates Lane 3: human brain lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P09544
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 40418 Da
Additional Information
Gene ID 7472
Other Names Protein Wnt-2, Int-1-like protein 1, Int-1-related protein, IRP, WNT2, INT1L1, IRP
Target/Specificity This WNT2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 254-287 amino acids from the Central region of human WNT2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWNT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WNT2
Synonyms INT1L1, IRP
Function Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20018874). Functions as a upstream regulator of FGF10 expression. Plays an important role in embryonic lung development. May contribute to embryonic brain development by regulating the proliferation of dopaminergic precursors and neurons (By similarity).
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted
Tissue Location Expressed in brain in the thalamus, in fetal and adult lung and in placenta.
Research Areas
Citations (0)
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Background

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.

References

Wainwright B.J.,et al.EMBO J. 7:1743-1748(1988).
Farrall M.,et al.Submitted (APR-1988) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 424:157-164(2003).

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$ 140.00
Cat# AP21468c
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