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SH3GL2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SH3GL2 Antibody (C-term) AP21467b
    All lanes : Anti-SH3GL2 Antibody (C-term) at 1:2000 dilution Lane 1: PC-12 whole cell lysates Lane 2: human brain lysates Lane 3: human cerebellum lysates Lane 4: F9 whole cell lysates Lane 5: Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q99962
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 39962 Da
Additional Information
Gene ID 6456
Other Names Endophilin-A1, EEN-B1, Endophilin-1, SH3 domain protein 2A, SH3 domain-containing GRB2-like protein 2, SH3GL2, CNSA2, SH3D2A
Target/Specificity This SH3GL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 245-279 amino acids from the C-terminal region of human SH3GL2.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSH3GL2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH3GL2
Synonyms CNSA2, SH3D2A
Function Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Required for BDNF-dependent dendrite outgrowth. Cooperates with SH3GL2 to mediate BDNF-NTRK2 early endocytic trafficking and signaling from early endosomes.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O35179}. Membrane {ECO:0000250|UniProtKB:O35179}; Peripheral membrane protein {ECO:0000250|UniProtKB:O35179}. Early endosome {ECO:0000250|UniProtKB:Q62420}. Presynapse {ECO:0000250|UniProtKB:O35179}
Tissue Location Brain, mostly in frontal cortex. Expressed at high level in fetal cerebellum
Research Areas
Citations (0)
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Background

Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature.

References

Giachino C.,et al.Genomics 41:427-434(1997).
So C.W.,et al.Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 140.00
Cat# AP21467b
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