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OXCT1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - OXCT1 Antibody (Center) AP21464c
    All lanes : Anti-OXCT1 Antibody (Center) at 1:2000 dilution Lane 1: Jurkat whole cell lysates Lane 2: HepG2 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: human heart lysates Lane 5: mouse heart lysates Lane 6: rat heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 56 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P55809
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 56158 Da
Additional Information
Gene ID 5019
Other Names Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, 3-oxoacid CoA-transferase 1, Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, SCOT-s, OXCT1, OXCT, SCOT
Target/Specificity This OXCT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-306 amino acids from the Central region of human OXCT1.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOXCT1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OXCT1
Synonyms OXCT, SCOT
Function Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl- CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (PubMed:10964512). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl- CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity).
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:B2GV06}.
Tissue Location Abundant in heart, followed in order by brain, kidney, skeletal muscle, and lung, whereas in liver it is undetectable Expressed (at protein level) in all tissues (except in liver), most abundant in myocardium, then brain, kidney, adrenal glands, skeletal muscle and lung; also detectable in leukocytes and fibroblasts
Research Areas
Citations ( 0 )

Background

Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.

References

Kassovska-Bratinova S.,et al.Am. J. Hum. Genet. 59:519-528(1996).
Fukao T.,et al.Genomics 68:144-151(2000).
Schmutz J.,et al.Nature 431:268-274(2004).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Reymond M.A.,et al.Submitted (FEB-1997) to UniProtKB.

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$ 140.00
Cat# AP21464c
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