Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
(Mouse) Pou5f1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P20263 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 38216 Da |
| Gene ID | 18999 |
|---|---|
| Other Names | POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3 |
| Target/Specificity | This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-119 amino acids from the N-terminal region of Mouse Pou5f1. |
| Dilution | WB~~1:2000 IHC-P~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | (Mouse) Pou5f1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Pou5f1 |
|---|---|
| Synonyms | Oct-3, Oct-4, Otf-3, Otf3 |
| Function | Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3') (PubMed:1690859, PubMed:17525163, PubMed:1967980, PubMed:1972777, PubMed:23376973). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:15863505, PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:1972777, PubMed:19740739, PubMed:23376973, PubMed:29153991, PubMed:32703285). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q01860}. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE- ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163, ECO:0000269|PubMed:29153991} Note=Expressed in a diffuse and slightly punctuate pattern (By similarity). Colocalizes with MAPK8 and MAPK9 in the nucleus (PubMed:29153991). {ECO:0000250|UniProtKB:Q01860, ECO:0000269|PubMed:29153991} |
| Tissue Location | Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
References
Rosner M.H.,et al.Nature 345:686-692(1990).
Schoeler H.R.,et al.Nature 344:435-439(1990).
Okamoto K.,et al.Cell 60:461-472(1990).
Okazawa H.,et al.EMBO J. 10:2997-3005(1991).
Tsuruzoe S.,et al.Biochem. Biophys. Res. Commun. 351:920-926(2006).
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
