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(Mouse) Eras Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - (Mouse) Eras Antibody (C-term) AP21303b
    All lanes : Anti-Eras Antibody (C-term) at 1:2000 dilution Lane 1: mouse heart lysates Lane 2: rat heart lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - (Mouse) Eras Antibody (C-term) AP21303b
    Anti-Eras Antibody (C-term)at 1:2000 dilution + mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q7TN89
Reactivity Mouse, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 24321 Da
Additional Information
Gene ID 353283
Other Names GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras
Target/Specificity This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 202-236 amino acids from the C-terminal region of Mouse Eras.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Eras Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Eras
Function Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Tissue Location Expressed in several undifferentiated mouse embryonic stem cell lines.
Research Areas
Citations (0)
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Background

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells.

References

Takahashi K.,et al.Nature 423:541-545(2003).
Carninci P.,et al.Science 309:1559-1563(2005).

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$ 182.50
$ 70.00
Cat# AP21303b
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