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Htr2a Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P35363 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 52842 Da |
| Gene ID | 15558 |
|---|---|
| Other Names | 5-hydroxytryptamine receptor 2A, 5-HT-2, 5-HT-2A, Serotonin receptor 2A, Htr2a, Htr2 |
| Target/Specificity | This Htr2a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human Htr2a. |
| Dilution | WB~~1:2000 IHC-P~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Htr2a Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Htr2a |
|---|---|
| Synonyms | Htr2 |
| Function | G-protein coupled receptor for 5-hydroxytryptamine (serotonin) (PubMed:11960784, PubMed:16873667, PubMed:17270739, PubMed:18297054, PubMed:21645528, PubMed:23129762, PubMed:23346101). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4- iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD) (By similarity). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors (By similarity). HTR2A is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively (By similarity). Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways (PubMed:18297054). Affects neural activity, perception, cognition and mood (PubMed:18297054). Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances (PubMed:16873667, PubMed:17270739, PubMed:18297054, PubMed:23129762). Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction (PubMed:11960784, PubMed:23346101). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Cell projection, axon {ECO:0000250|UniProtKB:P14842}. Cytoplasmic vesicle. Membrane, caveola {ECO:0000250|UniProtKB:P14842}. Presynapse {ECO:0000250|UniProtKB:P14842} |
| Tissue Location | Detected in neurons in brain cortex. Detected in adult intestine, especially in mucosal epithelium, longitudinal and circular layers of muscularis externa and myenteric plexuses. Highly expressed in Paneth cells, and detected at lower levels in enterocytes (at protein level). Detected in neurons in the brain cortex |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5- dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca(2+) ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.
References
Yang W.,et al.J. Neurosci. Res. 33:196-204(1992).
Fiorica-Howells E.,et al.Am. J. Physiol. 282:G877-G893(2002).
Becamel C.,et al.J. Biol. Chem. 279:20257-20266(2004).
Weisstaub N.V.,et al.Science 313:536-540(2006).
Gonzalez-Maeso J.,et al.Neuron 53:439-452(2007).
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