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DRD5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - DRD5 Antibody (C-term) AP21293b
    Anti-DRD5 Antibody (C-term)at 1:2000 dilution + human ovary lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • FC - DRD5 Antibody (C-term) AP21293b
    Overlay histogram showing U-87 MG cells stained with AP21293b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21293b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P21918
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 52951 Da
Additional Information
Gene ID 1816
Other Names D(1B) dopamine receptor, D(5) dopamine receptor, D1beta dopamine receptor, Dopamine D5 receptor, DRD5, DRD1B, DRD1L2
Target/Specificity This DRD5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 421-456 amino acids from the C-terminal region of human DRD5.
Dilution WB~~1:2000
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDRD5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DRD5
Synonyms DRD1B, DRD1L2
Function Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Cellular Location Cell membrane; Multi-pass membrane protein.
Tissue Location Neuron-specific, localized primarily within limbic regions of the brain.
Research Areas
Citations (0)
citation

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Background

Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

References

Sunahara R.K.,et al.Nature 350:614-619(1991).
Grandy D.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:9175-9179(1991).
Weinshank R.L.,et al.J. Biol. Chem. 266:22427-22435(1991).
Puhl H.L. III,et al.Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 365.00
$ 140.00
Cat# AP21293b
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