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PPAP2B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - PPAP2B Antibody (N-term) AP21245a
    All lanes : Anti-PPAP2B Antibody (N-term) at 1:2000 dilution Lane 1: SW620 whole cell lysates Lane 2: human placenta lysates Lane 3: human kidney lysates Lane 4: human lung lysates Lane 5: rat liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O14495
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 35116 Da
Additional Information
Gene ID 8613
Other Names Lipid phosphate phosphohydrolase 3, PAP2-beta, Phosphatidate phosphohydrolase type 2b, Phosphatidic acid phosphatase 2b, PAP-2b, PAP2b, Vascular endothelial growth factor and type I collagen-inducible protein, VCIP, PPAP2B, LPP3
Target/Specificity This PPAP2B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 3~37 amino acids from the N-terminal region of human PPAP2B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPAP2B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLPP3 (HGNC:9229)
Synonyms LPP3, PPAP2B
Function Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1- phosphate/C1P (PubMed:27694435, PubMed:9607309, PubMed:9705349). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)- ethanolamine phosphate, a potential physiological compound (PubMed:9607309). Has both an extracellular and an intracellular phosphatase activity, allowing the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed:23591818, PubMed:27694435, PubMed:9607309). Through the dephosphorylation of extracellular sphingosine-1-phosphate and the regulation of its extra- and intracellular availability, plays a role in vascular homeostasis, regulating endothelial cell migration, adhesion, survival, proliferation and the production of pro-inflammatory cytokines (PubMed:27694435). By maintaining the appropriate levels of this lipid in the cerebellum, also ensure its proper development and function (By similarity). Through its intracellular lipid phosphatase activity may act in early compartments of the secretory pathway, regulating the formation of Golgi to endoplasmic reticulum retrograde transport carriers (PubMed:23591818).
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Basolateral cell membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Endoplasmic reticulum membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Endoplasmic reticulum-Golgi intermediate compartment membrane; Multi- pass membrane protein {ECO:0000250|UniProtKB:P97544}. Golgi apparatus membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Membrane raft; Multi-pass membrane protein {ECO:0000250|UniProtKB:P97544}. Note=Cycles between the endoplasmic reticulum and the Golgi.
Tissue Location Ubiquitously expressed (PubMed:12660161, PubMed:9305923). Highly expressed in heart and placenta (PubMed:9305923).
Research Areas
Citations (0)
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Background

Catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG). In addition it hydrolyzes lysophosphatidic acid (LPA), ceramide-1-phosphate (C-1-P) and sphingosine-1- phosphate (S-1-P). The relative catalytic efficiency is LPA = PA > C-1-P > S-1-P. May be involved in cell adhesion and in cell-cell interactions.

References

Kai M.,et al.J. Biol. Chem. 272:24572-24578(1997).
Roberts R.,et al.J. Biol. Chem. 273:22059-22067(1998).
Humtsoe J.O.,et al.EMBO J. 22:1539-1554(2003).
Leung D.W.,et al.Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Genome Res. 7:353-358(1997).

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$ 365.00
$ 140.00
Cat# AP21245a
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