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IL24 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - IL24 Antibody AP21241a
    All lanes : Anti-IL24 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: K562 whole cell lysates Lane 4: A431 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - IL24 Antibody AP21241a
    AP21241a staining IL24 in human melanoma sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - IL24 Antibody AP21241a
    Overlay histogram showing U-2 OS cells stained with AP21241a (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP21241a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q13007
Reactivity Human
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 23825 Da
Additional Information
Gene ID 11009
Other Names Interleukin-24, IL-24, Melanoma differentiation-associated gene 7 protein, MDA-7, Suppression of tumorigenicity 16 protein, IL24, MDA7, ST16
Target/Specificity This IL24 antibody is generated from a rabbit immunized with a recombinant protein.
Dilution WB~~1:2000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIL24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IL24
Synonyms MDA7, ST16
Function Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis (PubMed:25168428, PubMed:27687232). Possesses antiviral functions and induces the type I interferon response during influenza infection (PubMed:27687232). Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1 (PubMed:11706020, PubMed:30111632). In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1 (PubMed:25168428). Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway- mediated stress signal (By similarity). In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2 (By similarity).
Cellular Location Secreted.
Tissue Location Up-regulated in melanoma cells induced to terminally differentiate
Research Areas
Citations (0)
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Background

Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.

References

Jiang H.,et al.Oncogene 11:2477-2486(1995).
Peat J.,et al.Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases.
Huang E.Y.,et al.Oncogene 20:7051-7063(2001).
Allen M.,et al.J. Invest. Dermatol. 123:583-588(2004).
Allen M.,et al.Int. J. Immunogenet. 32:375-378(2005).

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$ 365.00
$ 140.00
Cat# AP21241a
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