Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
COVID19
>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   (Mouse) Ihh Antibody (N-term)   

(Mouse) Ihh Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
crown
Products labeled with a "crown" meet aggressive quality standards. Learn more.
  • WB - (Mouse) Ihh Antibody (N-term) AP21224a
    Anti-Ihh Antibody (N-term) at 1:2000 dilution + mouse kidney lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - (Mouse) Ihh Antibody (N-term) AP21224a
    AP21224a staining (Mouse) Ihh in mouse kidney sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - (Mouse) Ihh Antibody (N-term) AP21224a
    Overlay histogram showing Jurkat cells stained with AP21224a (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP12735b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
  • detail
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession P97812
Reactivity Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 45485 Da
Additional Information
Gene ID 16147
Other Names Indian hedgehog protein, IHH, HHG-2, Indian hedgehog protein N-product, Indian hedgehog protein C-product, Ihh
Target/Specificity This mouse Ihh antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 57-90 amino acids from the N-terminal region of mouse Ihh.
Dilution WB~~1:2000
IHC-P~~1:25
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Ihh Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Ihh {ECO:0000312|MGI:MGI:96533}
Function [Indian hedgehog protein]: The C-terminal part of the indian hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity (By similarity). Both activities result in the cleavage of the full-length protein into two parts followed by the covalent attachment of a cholesterol moiety to the C- terminal of the newly generated N-product (By similarity). Both activities occur in the reticulum endoplasmic (By similarity). Plays a role in hedgehog paracrine signaling. Associated with the very-low- density lipoprotein (VLDL) particles to function as a circulating morphogen for endothelial cell integrity maintenance (By similarity).
Cellular Location [Indian hedgehog protein N-product]: Cell membrane {ECO:0000250|UniProtKB:Q14623}; Lipid-anchor {ECO:0000250|UniProtKB:Q62226}. Note=The N-product remains associated with the cell surface. {ECO:0000250|UniProtKB:Q15465}
Tissue Location In the adult kidney, found in proximal convoluted and proximal straight tubule.
Research Areas
Citations (0)
citation

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abcepta antibody to
info@abcepta.com, and receive a free "I Love Antibodies" mug.

Background

Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP).

References

Valentini R.P.,et al.J. Biol. Chem. 272:8466-8473(1997).
Echelard Y.,et al.Cell 75:1417-1430(1993).
St Jacques B.,et al.Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
Chang D.T.,et al.Development 120:3339-3353(1994).

FeedBack
Abcepta welcomes feedback from its customers.

If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abcepta.com.

$ 365.00
$ 140.00
Cat# AP21224a
Size:
Quantity:
Availability: Out of Stock
Bulk Size

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaCentral AmericaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSouth KoreaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamWorldwideOthers
Abcepta, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Terms & Conditions
"