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DNASE1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DNASE1 Antibody (Center) AP21123a
    All lanes : Anti-DNASE1 Antibody (Center) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P-Leica - DNASE1 Antibody (Center) AP21123a
    Immunohistochemical analysis of paraffin-embedded Human stomach tissue using AP21123A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P-Leica, WB, E
Primary Accession P24855
Other Accession P00639
Reactivity Human, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 31434 Da
Additional Information
Gene ID 1773
Other Names Deoxyribonuclease-1, Deoxyribonuclease I, DNase I, Dornase alfa, DNASE1, DNL1, DRNI
Target/Specificity This DNASE1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 87-121 amino acids from the Central region of human DNASE1.
Dilution WB~~1:1000
IHC-P-Leica~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNASE1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNASE1 (HGNC:2956)
Synonyms DNL1, DRNI
Function Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:11241278, PubMed:2251263, PubMed:2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
Cellular Location Secreted. Zymogen granule. Nucleus envelope. Note=Secretory protein, stored in zymogen granules and found in the nuclear envelope
Tissue Location Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva
Research Areas
Citations (0)
citation

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Background

Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization (By similarity).

References

Shak S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:9188-9192(1990).
Yasuda T.,et al.Ann. Hum. Genet. 59:1-15(1995).
Oliveri M.,et al.Eur. J. Immunol. 31:743-751(2001).
Kominato Y.,et al.FEBS J. 273:3094-3105(2006).
Martin J.,et al.Nature 432:988-994(2004).

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$ 182.50
$ 70.00
Cat# AP21123a
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