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WDR82 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - WDR82 Antibody (N-term) AP20977b
    All lanes : Anti-WDR82 Antibody (N-term) at 1:1000 dilution Lane 1: F9 whole cell lysate Lane 2:NIH/3T3 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - WDR82 Antibody (N-term) AP20977b
    AP20977b staining WDR82 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/250) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • FC - WDR82 Antibody (N-term) AP20977b
    Overlay histogram showing HepG2 cells stained with AP20977b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20977b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P, E
Primary Accession Q6UXN9
Other Accession Q8BFQ4, Q6NV31, Q5ZMV7, Q58E77, Q640J6
Reactivity Human, Mouse, Zebrafish
Predicted Xenopus, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35079 Da
Antigen Region 50-85 aa
Additional Information
Gene ID 80335
Other Names WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A
Target/Specificity This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 50-85 amino acids from the N-terminal region of human WDR82.
Dilution WB~~1:1000
IHC-P~~1:250
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWDR82 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WDR82 {ECO:0000303|PubMed:17998332, ECO:0000312|HGNC:HGNC:28826}
Function Regulatory component of the SET1/COMPASS complex implicated in the tethering of this complex to transcriptional start sites of active genes (PubMed:17998332, PubMed:18838538, PubMed:20516061). Facilitates histone H3 'Lys-4' methylation (H3K4me) via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) (PubMed:17998332, PubMed:18838538). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase (PubMed:20516061). Together with ZC3H4, but independently of the SET1 complex, part of a transcription termination checkpoint that promotes transcription termination of long non-coding RNAs (lncRNAs) (PubMed:33767452, PubMed:33913806). The transcription termination checkpoint is activated by the inefficiently spliced first exon of lncRNAs and promotes transcription termination of lncRNAs and their subsequent degradation by the exosome (PubMed:33767452).
Cellular Location Nucleus. Chromosome {ECO:0000250|UniProtKB:Q8BFQ4}. Note=Associates with chromatin (PubMed:20516061). Recruited at sites of high RNA polymerase II occupancy (By similarity). {ECO:0000250|UniProtKB:Q8BFQ4, ECO:0000269|PubMed:20516061}
Research Areas
Citations (0)
citation

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Background

Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 'Lys-4' methylation via recruitment of the SETD1A or SETD1B to the 'Ser-5' phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.

References

Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Lee J.-H.,et al.J. Biol. Chem. 280:41725-41731(2005).
Higa L.A.,et al.Nat. Cell Biol. 8:1277-1283(2006).

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$ 365.00
$ 140.00
Cat# AP20977b
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