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CD34 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - CD34 Antibody (C-term) AP20932c
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human haemangioma tissue labeling CD34 with AP20932c at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on human haemangioma tissue. The nuclear counter stain is DAPI (blue).
    detail
  • WB - CD34 Antibody (C-term) AP20932c
    Western blot analysis of lysate from KG-1 cell line, using CD34 Antibody (C-term)(Cat. #AP20932c). AP20932c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P28906
Other Accession Q64314
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 40716 Da
Additional Information
Gene ID 947
Other Names Hematopoietic progenitor cell antigen CD34, CD34, CD34
Target/Specificity This CD34 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 320-354 amino acids from the C-terminal region of human CD34.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD34 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD34
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
Cellular Location Membrane; Single-pass type I membrane protein.
Tissue Location Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues
Research Areas
Citations (0)
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Background

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

References

Simmons D.L.,et al.J. Immunol. 148:267-271(1992).
Satterthwaite A.B.,et al.Genomics 12:788-794(1992).
Nakamura Y.,et al.Exp. Hematol. 21:236-242(1993).
Freund D.,et al.Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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$ 365.00
$ 140.00
Cat# AP20932c
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