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Mouse Hmga2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - Mouse Hmga2 Antibody (C-term) AP20905c
    Fluorescent image of C2C12cells stained with Mouse Hmga2 Antibody (C-term)(Cat#AP20905c). AP20905c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - Mouse Hmga2 Antibody (C-term) AP20905c
    Western blot analysis of lysates from HepG2, NCCIT, mouse NIH/3T3, mouse F9 cell line (from left to right), using Hmga2 Antibody (C-term)(Cat. #AP20905c). AP20905c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession P52927
Other Accession P52926
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11819 Da
Additional Information
Gene ID 15364
Other Names High mobility group protein HMGI-C, High mobility group AT-hook protein 2, Hmga2, Hmgic
Target/Specificity This Mouse Hmga2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 72-105 amino acids from the C-terminal region of Mouse Hmga2.
Dilution IF~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Hmga2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hmga2
Synonyms Hmgic
Function Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes. Plays a role in postnatal myogenesis, is involved in satellite cell activation (PubMed:27446912). Positively regulates IGF2 expression through PLAG1 and in a PLAG1-independent manner (By similarity).
Cellular Location Nucleus.
Tissue Location Expressed in mitotic spermatogonia, meiotic spermatocytes, and postmeiotic round spermatids (at protein level) (PubMed:14668482). Expressed in embryonic myogenic progenitor cells (PubMed:27446912).
Research Areas
Citations (0)
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Background

Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. Plays an important role in chromosome condensation during the meiotic G2/M transition of spermatocytes.

References

Manfioletti G.,et al.Nucleic Acids Res. 19:6793-6797(1991).
Manfioletti G.,et al.Gene 167:249-253(1995).
Zhou X.,et al.Nucleic Acids Res. 24:4071-4077(1996).
Carninci P.,et al.Science 309:1559-1563(2005).
Di Agostino S.,et al.Mol. Biol. Cell 15:1224-1232(2004).

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$ 365.00
$ 140.00
Cat# AP20905c
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