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Histone H2A.X (Ser139) Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P16104 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 15145 Da |
| Gene ID | 3014 |
|---|---|
| Other Names | Histone H2AX, H2a/x, Histone H2AX, H2AFX, H2AX |
| Target/Specificity | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 134-163 amino acids from human. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Histone H2A.X (Ser139) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | H2AX (HGNC:4739) |
|---|---|
| Function | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. |
| Cellular Location | Nucleus. Chromosome |
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Provided below are standard protocols that you may find useful for product applications.
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
References
Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999).
Paull T.T.,et al.Curr. Biol. 10:886-895(2000).
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