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CYB5R3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CYB5R3 Antibody (Center) AP20631b
    Western blot analysis of lysates from A431, Hela, HepG2 cell line , mouse liver and rat liver tissue lysate(from left to right), using CYB5R3 Antibody (Center)(Cat. #AP20631b). AP20631b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P00387
Other Accession P20070, P83686, Q9DCN2, Q60HG4, P07514
Reactivity Human, Mouse, Rat
Predicted Bovine, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 34235 Da
Additional Information
Gene ID 1727
Other Names NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1, NADH-cytochrome b5 reductase 3 membrane-bound form, NADH-cytochrome b5 reductase 3 soluble form, CYB5R3, DIA1
Target/Specificity This CYB5R3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-144 amino acids from the Central region of human CYB5R3
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R3 (HGNC:2873)
Synonyms DIA1
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Cellular Location [Isoform 1]: Endoplasmic reticulum membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}. Mitochondrion outer membrane; Lipid-anchor {ECO:0000250|UniProtKB:P20070}; Cytoplasmic side {ECO:0000250|UniProtKB:P20070}
Tissue Location [Isoform 2]: Expressed at late stages of erythroid maturation.
Research Areas
Citations (0)
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Background

Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.

References

Tomatsu S.,et al.Gene 80:353-361(1989).
Voice M.W.,et al.Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
Yoon B.,et al.Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases.
Lan F.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP20631b
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