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DHFR Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC-P, WB, E |
|---|---|
| Primary Accession | P00374 |
| Other Accession | P00377, P00375, P00376 |
| Reactivity | Human, Mouse, Rat |
| Predicted | Bovine, Pig |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 21453 Da |
| Gene ID | 1719 |
|---|---|
| Other Names | Dihydrofolate reductase, DHFR |
| Target/Specificity | This DHFR antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-30 amino acids from the N-terminal region of human DHFR. |
| Dilution | WB~~1:1000 IHC-P~~1:25 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DHFR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DHFR |
|---|---|
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2. |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P00375}. Cytoplasm {ECO:0000250|UniProtKB:P00375} |
| Tissue Location | Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood Expression is higher in the adult brain than in the fetal brain |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1.
References
Chen M.-J.,et al.J. Biol. Chem. 259:3933-3943(1984).
Masters J.N.,et al.Gene 21:59-63(1983).
Yang J.K.,et al.J. Mol. Biol. 176:169-187(1984).
Schmutz J.,et al.Nature 431:268-274(2004).
Banka S.,et al.Am. J. Hum. Genet. 88:216-225(2011).
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