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GSTM4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GSTM4 Antibody (N-term) AP20566a
    Western blot analysis of lysate from MCF-7 cell line, using GSTM4 Antibody (N-term)(Cat. #AP20566a). AP20566a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
    detail
  • WB - GSTM4 Antibody (N-term) AP20566a
    All lanes : Anti-GSTM4 Antibody (N-term) at 1:1000 dilution Lane 1: Human lung tissue lysate Lane 2: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - GSTM4 Antibody (N-term) AP20566a
    All lanes : Anti-GSTM4 Antibody (N-term) at 1:2000 dilution Lane 1: Human liver tissue lysate Lane 2: MCF-7 whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q03013
Other Accession P46439, Q9TSM4, P28161, P04905, Q9TSM5, P09488, Q9N0V4
Reactivity Human
Predicted Bovine, Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 25561 Da
Additional Information
Gene ID 2948
Other Names Glutathione S-transferase Mu 4, GST class-mu 4, GST-Mu2, GSTM4-4, GSTM4
Target/Specificity This GSTM4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 16-50 amino acids from the N-terminal region of human GSTM4.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSTM4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSTM4
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:8203914, PubMed:8373352). Catalyzes the conjugation of leukotriene A4 with reduced glutathione (GSH) to form leukotriene C4 (PubMed:27791009). Can also catalyze the transfer of a glutathionyl group from glutathione (GSH) to 13(S),14(S)-epoxy-docosahexaenoic acid to form maresin conjugate in tissue regeneration 1 (MCTR1), a bioactive lipid mediator that possess potent anti-inflammatory and proresolving actions (PubMed:27791009).
Cellular Location Cytoplasm.
Tissue Location Expressed in a wide variety of tissues.
Research Areas
Citations (0)
citation

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Background

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1- chloro-2,4-dinitrobenzene.

References

Comstock K.E.,et al.J. Biol. Chem. 268:16958-16965(1993).
Zhong S.,et al.Biochem. J. 291:41-50(1993).
Ross V.L.,et al.Biochem. J. 294:373-380(1993).
Denson J.,et al.Gene 379:148-155(2006).
Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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$ 365.00
$ 140.00
Cat# AP20566a
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