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Rat Atp1a1 Antibody (N-term S23)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - Rat Atp1a1 Antibody (N-term S23) AP20549a
    Western blot analysis of lysate from PC-12 cell line,using Rat Atp1a1 Antibody (S23)(Cat. #AP20549a).AP20549a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysate at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P06685
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 113054 Da
Additional Information
Gene ID 24211
Other Names Sodium/potassium-transporting ATPase subunit alpha-1, Na(+)/K(+) ATPase alpha-1 subunit, Sodium pump subunit alpha-1, Atp1a1
Target/Specificity This Rat Atp1a1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from Rat Atp1a1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRat Atp1a1 Antibody (N-term S23) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Atp1a1
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (PubMed:30388404). Could also be part of an osmosensory signaling pathway that senses body-fluid sodium levels and controls salt intake behavior as well as voluntary water intake to regulate sodium homeostasis (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q8VDN2}; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:P05023}; Multi-pass membrane protein. Cell projection, axon Melanosome {ECO:0000250|UniProtKB:P05023}
Tissue Location Expressed in the central nervous system, in most motor and sensory axons of the ventral and dorsal roots, as well as in the large motor neurons of the ventral horn (at protein level)
Research Areas
Citations (0)
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Background

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

References

Shull G.E.,et al.Biochemistry 25:8125-8132(1986).
Hara Y.,et al.J. Biochem. 102:43-58(1987).
Herrera V.L.M.,et al.J. Cell Biol. 105:1855-1865(1987).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Schneider J.W.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:6357-6361(1985).

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$ 365.00
$ 140.00
Cat# AP20549a
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