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VAMP8 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - VAMP8 Antibody (N-term) AP20523A
    VAMP8 Antibody (N-term) (Cat. #AP20523a) western blot analysis in Hela,293T cell line lysates (35ug/lane).This demonstrates the VAMP8 antibody detected the VAMP8 protein (arrow).
    detail
  • IHC-P - VAMP8 Antibody (N-term) AP20523A
    Immunohistochemical analysis of paraffin-embedded H. stomach section using VAMP8 Antibody (N-term)(Cat#AP20523A). AP20523A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • IHC-P - VAMP8 Antibody (N-term) AP20523A
    Formalin-fixed and paraffin-embedded H.placenta tissue reacted with VAMP8 Antibody (N-term) (Cat#AP20523a).
    detail
  • IHC-P - VAMP8 Antibody (N-term) AP20523A
    Formalin-fixed and paraffin-embedded H.spleen tissue reacted with VAMP8 Antibody (N-term) (Cat#AP20523a).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9BV40
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 11438 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 8673
Other Names Vesicle-associated membrane protein 8, VAMP-8, Endobrevin, EDB, VAMP8
Target/Specificity This VAMP8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VAMP8.
Dilution WB~~1:1000
IHC-P~~1:100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAMP8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAMP8 {ECO:0000303|PubMed:12130530}
Function SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane via its interaction with the STX17-SNAP29 binary t- SNARE complex (PubMed:23217709, PubMed:25686604). Also required for dense-granule secretion in platelets (PubMed:12130530). Also plays a role in regulated enzyme secretion in pancreatic acinar cells (By similarity). Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells (By similarity). Involved in the homotypic fusion of early and late endosomes (By similarity). Participates also in the activation of type I interferon antiviral response through a TRIM6-dependent mechanism (PubMed:31694946).
Cellular Location Lysosome membrane; Single-pass type IV membrane protein. Early endosome membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Cell membrane {ECO:0000250|UniProtKB:O70404}; Single-pass type IV membrane protein. Zymogen granule membrane {ECO:0000250|UniProtKB:O70404}; Single-pass type IV membrane protein. Note=Perinuclear vesicular structures of the early and late endosomes, coated pits, and trans-Golgi (By similarity) Sub-tight junctional domain in retinal pigment epithelium cells Midbody region during cytokinesis. Lumenal oriented, apical membranes of nephric tubular cell (By similarity). Cycles through the apical but not through the basolateral plasma membrane (By similarity). Apical region of acinar cells; in zymogen granule membranes (By similarity) {ECO:0000250|UniProtKB:Q9WUF4}
Tissue Location Platelets..
Research Areas
Citations (0)
citation

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Background

Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes (By similarity).

References

Wong S.H., et al. Mol. Biol. Cell 9:1549-1563(1998).
Kalnine N., et al. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W., et al. Nature 434:724-731(2005).
Polgar J., et al. Blood 100:1081-1083(2002).

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$ 365.00
$ 140.00
Cat# AP20523A
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