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R Cdk4 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - R Cdk4 Antibody (C-term) AP20515b
    Fluorescent image of MCF-7 cells stained with (Rat) Cdk4 Antibody (C-term)(Cat#AP20515B). AP20515B was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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  • WB - R Cdk4 Antibody (C-term) AP20515b
    Rat Cdk4 Antibody (C-term) (Cat. #AP20515b) western blot analysis in rat lung tissue lysates (35ug/lane).This demonstrates the rat Cdk4 antibody detected the rat Cdk4 protein (arrow).
    detail
  • IHC-P - R Cdk4 Antibody (C-term) AP20515b
    Immunohistochemical analysis of paraffin-embedded R. brain section using R Cdk4 Antibody (C-term)(Cat#AP20515B). AP20515B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • IHC-P - R Cdk4 Antibody (C-term) AP20515b
    Immunohistochemical analysis of paraffin-embedded rat small intestine section using Rat Cdk4 Antibody (C-term)(Cat#AP20515B). AP20515B was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession P35426
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33799 Da
Antigen Region 272-303 aa
Additional Information
Gene ID 94201
Other Names Cyclin-dependent kinase 4, Cell division protein kinase 4, PSK-J3, Cdk4
Target/Specificity This Rat Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-303 amino acids from the C-terminal region of rat Cdk4.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsR Cdk4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdk4
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P11802}. Nucleus {ECO:0000250|UniProtKB:P11802}. Nucleus membrane {ECO:0000250|UniProtKB:P11802}. Note=Cytoplasmic when non-complexed Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus (By similarity). {ECO:0000250|UniProtKB:P11802}
Tissue Location Expressed in fetal and adult lung. Also expressed in brain, heart, liver, skeletal muscle and testes
Research Areas
Citations (0)
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Background

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

References

Cho F.S., et al. Biochem. Biophys. Res. Commun. 191:860-865(1993).

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$ 365.00
$ 140.00
Cat# AP20515b
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