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SLC25A25 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SLC25A25 Antibody (N-term) AP20508a
    SLC25A25 Antibody (N-term) (Cat. #AP20508a) western blot analysis in U-937 cell line and rat cerebellum tissue lysates (35ug/lane).This demonstrates the SLC25A25 antibody detected the SLC25A25 protein (arrow).
    detail
  • IHC-P - SLC25A25 Antibody (N-term) AP20508a
    Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using SLC25A25 Antibody (N-term)(Cat#AP20508A). AP20508A was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q6KCM7
Other Accession Q7ZYD5, Q8K3P6, A2ASZ8, Q0V7M4, A2CEQ0
Reactivity Human, Rat
Predicted Zebrafish, Bovine, Mouse, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52663 Da
Antigen Region 63-93 aa
Additional Information
Gene ID 114789
Other Names Calcium-binding mitochondrial carrier protein SCaMC-2, Mitochondrial ATP-Mg/Pi carrier protein 3, Mitochondrial Ca(2+)-dependent solute carrier protein 3, Small calcium-binding mitochondrial carrier protein 2, Solute carrier family 25 member 25, SLC25A25, APC3, KIAA1896, MCSC3, SCAMC2
Target/Specificity This SLC25A25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-93 amino acids from the N-terminal region of human SLC25A25.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC25A25 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC25A25 (HGNC:20663)
Function Electroneutral antiporter that most probably mediates the transport of adenyl nucleotides through the inner mitochondrial membrane. Originally identified as an ATP-magnesium/inorganic phosphate antiporter, it could have a broader specificity for adenyl nucleotides. By regulating the mitochondrial matrix adenyl nucleotide pool could adapt to changing cellular energetic demands and indirectly regulate adenyl nucleotide-dependent metabolic pathways.
Cellular Location Mitochondrion inner membrane; Multi-pass membrane protein
Tissue Location Widely expressed. Expressed in fetal and adult liver, skeletal muscle, testis, ovary, hippocampus and caudate nucleus [Isoform 2]: Expression is restricted to kidney and lung.
Research Areas
Citations (0)
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Background

Calcium-dependent mitochondrial solute carrier. Mitochondrial solute carriers shuttle metabolites, nucleotides, and cofactors through the mitochondrial inner membrane. May act as a ATP-Mg/Pi exchanger that mediates the transport of Mg-ATP in exchange for phosphate, catalyzing the net uptake or efflux of adenine nucleotides into or from the mitochondria.

References

del Arco A., et al. J. Biol. Chem. 279:24701-24713(2004).
Fiermonte G., et al. J. Biol. Chem. 279:30722-30730(2004).
Nagase T., et al. DNA Res. 8:179-187(2001).
Clark H.F., et al. Genome Res. 13:2265-2270(2003).
Ota T., et al. Nat. Genet. 36:40-45(2004).

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$ 365.00
$ 140.00
Cat# AP20508a
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