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SOX2 Antibody (C265)
Purified Rabbit Polyclonal Antibody (Pab)
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- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IF, WB, FC, E |
|---|---|
| Primary Accession | P48431 |
| Other Accession | O42569, P48432, P48430, P54231 |
| Reactivity | Human |
| Predicted | Chicken, Mouse, Sheep, Xenopus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34310 Da |
| Antigen Region | 251-284 aa |
| Gene ID | 6657 |
|---|---|
| Other Names | Transcription factor SOX-2, SOX2 |
| Target/Specificity | This SOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-284 amino acids from human SOX2. |
| Dilution | IF~~1:100 WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SOX2 Antibody (C265) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SOX2 |
|---|---|
| Function | Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408). Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity). |
| Cellular Location | Nucleus speckle {ECO:0000250|UniProtKB:Q05066}. Cytoplasm {ECO:0000250|UniProtKB:Q05738}. Nucleus {ECO:0000250|UniProtKB:Q05738}. Note=Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (By similarity). Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity) Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus (By similarity). Nuclear import is facilitated by XPO4, a protein that usually acts as a nuclear export signal receptor (By similarity) {ECO:0000250|UniProtKB:Q05066, ECO:0000250|UniProtKB:Q05738} |
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Provided below are standard protocols that you may find useful for product applications.
Background
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. This protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation.
References
References for protein:
1.Remenyi, A., et al., Genes Dev. 17(16):2048-2059 (2003).
2.Wiebe, M.S., et al., J. Biol. Chem. 278(20):17901-17911 (2003).
3.Fantes, J., et al., Nat. Genet. 33(4):461-463 (2003).
4.Schepers, G.E., et al., Dev. Cell 3(2):167-170 (2002).
5.Kamachi, Y., et al., Trends Genet. 16(4):182-187 (2000).
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
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