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CNBP Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - CNBP Antibody (Center) AP20285c
    CNBP Antibody (Center) (Cat. #AP20285c) western blot analysis in MCF-7 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the CNBP antibody detected the CNBP protein (arrow).
    detail
  • IHC-P - CNBP Antibody (Center) AP20285c
    Immunohistochemical analysis of paraffin-embedded H. heart section using CNBP Antibody (Center)(Cat#AP20285c). AP20285c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P62633
Other Accession P62634, P53996, O42395, Q3T0Q6, NP_001120665.1
Reactivity Human, Rat
Predicted Bovine, Chicken, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 19463 Da
Antigen Region 91-118 aa
Additional Information
Gene ID 7555
Other Names Cellular nucleic acid-binding protein, CNBP, Zinc finger protein 9, CNBP, RNF163, ZNF9
Target/Specificity This CNBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-118 amino acids from the Central region of human CNBP.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCNBP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNBP (HGNC:13164)
Synonyms RNF163, ZNF9
Function Single-stranded DNA-binding protein that preferentially binds to the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', and thereby mediates transcriptional repression (PubMed:2562787). Has a role as transactivator of the Myc promoter (By similarity). Binds single-stranded RNA in a sequence-specific manner (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P53996}. Cytoplasm. Endoplasmic reticulum {ECO:0000250|UniProtKB:P53996} [Isoform 2]: Cytoplasm [Isoform 5]: Cytoplasm [Isoform 8]: Cytoplasm
Tissue Location Expressed in the liver, kidney, spleen, testis, lung, muscle and adrenal glands.
Research Areas
Citations (0)
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Background

This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA. The protein functions in cap-independent translation of ornithine decarboxylase mRNA, and may also function in sterol-mediated transcriptional regulation. A CCTG expansion in the first intron of this gene results in myotonic dystrophy type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

References

Catalli, C., et al. J Mol Diagn 12(5):601-606(2010)
Massa, R., et al. Neuropathol. Appl. Neurobiol. 36(4):275-284(2010)
Sammons, M.A., et al. PLoS ONE 5 (2), E9301 (2010) :
Lucchiari, S., et al. J. Neurol. Sci. 275 (1-2), 159-163 (2008) :
Auvinen, S., et al. Arthritis Rheum. 58(11):3627-3631(2008)

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$ 365.00
$ 140.00
Cat# AP20285c
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