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VAMP4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VAMP4 Antibody (Center) AP20234c
    VAMP4 Antibody (Center) (Cat. #AP20234c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O75379
Other Accession O70480, Q32L97, NP_003753.2
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 16397 Da
Antigen Region 61-90 aa
Additional Information
Gene ID 8674
Other Names Vesicle-associated membrane protein 4, VAMP-4, VAMP4
Target/Specificity This VAMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the Central region of human VAMP4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVAMP4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VAMP4
Function Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Cellular Location Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein Note=Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles
Research Areas
Citations (0)
citation

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Background

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.

References

Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Sabra, M.M., et al. Mol. Genet. Metab. 85(2):133-139(2005)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)
Hinners, I., et al. EMBO Rep. 4(12):1182-1189(2003)

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$ 365.00
$ 140.00
Cat# AP20234c
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