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MAP2 Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MAP2 Antibody AP2018e
    All lanes : Anti-MAP2 Antibody at 1:1000 dilution+ U-87 MG Cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 280kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - MAP2 Antibody AP2018e
    Western blot analysis of MAP2 Antibody (Cat. #AP2018e) in MCF-7 cell line lysates (35ug/lane). MAP2 (arrow) was detected using the purified Pab.
    detail
  • IHC-P - MAP2 Antibody AP2018e
    Formalin-fixed and paraffin-embedded mouse brain tissue reacted with MAP2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - MAP2 Antibody AP2018e
    MAP2 Antibody (Cat.#AP2018e) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, FC, E
Primary Accession P11137
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 199526 Da
Additional Information
Gene ID 4133
Other Names Microtubule-associated protein 2, MAP-2, MAP2
Target/Specificity This MAP2 antibody is generated from rabbits immunized with MAP2 recombinant protein.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Cellular Location Cytoplasm, cytoskeleton. Cell projection, dendrite {ECO:0000250|UniProtKB:P20357}
Research Areas
Citations (0)
citation

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Background

MAP2 encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.

References

Maddodi, N., et al. J. Biol. Chem. 285(1):242-254(2010)
Krishnan, C., et al. Am. J. Surg. Pathol. 33(11):1695-1704(2009)
Gambichler, T., et al. Am. J. Clin. Pathol. 131(5):710-714(2009)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Romeike, B.F., et al. Histopathology 54(4):504-505(2009)

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$ 365.00
$ 140.00
Cat# AP2018e
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