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GFAP Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GFAP Antibody (N-term) AP2017a
    Western blot analysis of GFAP (arrow) using GFAP Antibody (N-term) (Cat.#AP2017a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GFAP gene (Lane 2) (Origene Technologies).
    detail
  • WB - GFAP Antibody (N-term) AP2017a
    All lanes : Anti-GFAP Antibody (M1) at 1:8000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P14136
Other Accession NP_002046
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 49880 Da
Antigen Region 10-40 aa
Additional Information
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Target/Specificity This GFAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-40 amino acids from the N-terminal region of human GFAP.
Dilution WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFAP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GFAP
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Citations (0)
citation

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Background

GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system.

References

Quintanar, J.L., et al., Parasitol. Res. 90(4):261-263 (2003).
Shiroma, N., et al., Brain Dev. 25(2):116-121 (2003).
Nielsen, A.L., et al., J. Biol. Chem. 277(33):29983-29991 (2002).
Namekawa, M., et al., Ann. Neurol. 52(6):779-785 (2002).
Lopez-Egido, J., et al., Exp. Cell Res. 278(2):175-183 (2002).

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$ 182.50
$ 70.00
Cat# AP2017a
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