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CD73 (NT5E) Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 10
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P21589 |
| Other Accession | NP_002517 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 63368 Da |
| Antigen Region | 520-550 aa |
| Gene ID | 4907 |
|---|---|
| Other Names | 5'-nucleotidase, 5'-NT, Ecto-5'-nucleotidase, CD73, NT5E, NT5, NTE |
| Target/Specificity | This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 520-550 amino acids from the C-terminal region of human CD73 (NT5E). |
| Dilution | WB~~1:1000 IHC-P~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CD73 (NT5E) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NT5E |
|---|---|
| Synonyms | NT5, NTE |
| Function | Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). |
| Cellular Location | Cell membrane; Lipid-anchor, GPI-anchor |
Provided below are standard protocols that you may find useful for product applications.
Background
Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
References
Hashikawa, T., et al., J. Dent. Res. 82(11):888-892 (2003). Rosi, F., et al., Biomed. Pharmacother. 56(2):100-104 (2002). Misumi, Y., et al., Eur. J. Biochem. 191(3):563-569 (1990). Boyle, J.M., et al., Hum. Genet. 81(1):88-92 (1988). Kalsi, K., et al., Mol. Cell. Biochem. 232 (1-2), 113-119 (2002).
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