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TFIP11 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - TFIP11 Antibody (N-term) AP20105a
    TFIP11 Antibody (N-term) (Cat.# AP20105a) western blot analysis in Hela,K562 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the TFIP11 antibody detected the TFIP11 protein (arrow).
    detail
  • IHC-P - TFIP11 Antibody (N-term) AP20105a
    Immunohistochemical analysis of paraffin-embedded H. liver section using TFIP11 Antibody (N-term)(Cat#AP20105a). AP20105a was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q9UBB9
Other Accession A1XD95, NP_036275.1
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 96820 Da
Antigen Region 42-70 aa
Additional Information
Gene ID 24144
Other Names Tuftelin-interacting protein 11, Septin and tuftelin-interacting protein 1, STIP-1, TFIP11, STIP
Target/Specificity This TFIP11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the N-terminal region of human TFIP11.
Dilution WB~~1:1000
IHC-P~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFIP11 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFIP11
Synonyms STIP
Function Involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. Intron turnover seems to proceed through reactions in two lariat-intron associated complexes termed Intron Large (IL) and Intron Small (IS). In cooperation with DHX15 seems to mediate the transition of the U2, U5 and U6 snRNP- containing IL complex to the snRNP-free IS complex leading to efficient debranching and turnover of excised introns. May play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.
Cellular Location Cytoplasm. Nucleus. Note=In the nucleus localizes to unique speckle domains in close proximity to nuclear speckles and not identical to paraspeckles
Research Areas
Citations (0)
citation

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Background

TFIP11 is a nuclear speckle-localized protein that may play a role in spliceosome disassembly in Cajal bodies (Stanek et al., 2008 [PubMed 18367544]).

References

Saus, E., et al. J Psychiatr Res (2010) In press :
Tannukit, S., et al. Biochem. Biophys. Res. Commun. 390(3):1044-1050(2009)
Gratacos, M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
Stanek, D., et al. Mol. Biol. Cell 19(6):2534-2543(2008)
Patir, A., et al. Caries Res. 42(5):394-400(2008)

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$ 365.00
$ 140.00
Cat# AP20105a
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