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UCHL5 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UCHL5 Antibody (Center) AP20058c
    UCHL5 Antibody (Center) (Cat. #AP20058c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the UCHL5 antibody detected the UCHL5 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9Y5K5
Other Accession NP_057068.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 37607 Da
Antigen Region 136-164 aa
Additional Information
Gene ID 51377
Other Names Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, UCHL5, UCH37
Target/Specificity This UCHL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 136-164 amino acids from the Central region of human UCHL5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCHL5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UCHL5
Synonyms UCH37
Function Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.
Cellular Location Cytoplasm. Nucleus. Note=Associates with the proteasome 19S subunit in the cytoplasm. Associates with the INO80 complex in the nucleus
Research Areas
Citations (0)
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Background

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.

References

Nishio, K., et al. Biochem. Biophys. Res. Commun. 390(3):855-860(2009)
Yao, T., et al. Mol. Cell 31(6):909-917(2008)
Koulich, E., et al. Mol. Biol. Cell 19(3):1072-1082(2008)
Qiu, X.B., et al. EMBO J. 25(24):5742-5753(2006)
Hamazaki, J., et al. EMBO J. 25(19):4524-4536(2006)

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$ 182.50
$ 70.00
Cat# AP20058c
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