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AMPH Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AMPH Antibody (Center) AP19926C
    AMPH Antibody (Center) (Cat. #AP19926c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the AMPH antibody detected the AMPH protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P49418
Other Accession O08838, Q7TQF7, NP_647477.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 76257 Da
Antigen Region 140-168 aa
Additional Information
Gene ID 273
Other Names Amphiphysin, AMPH, AMPH1
Target/Specificity This AMPH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the Central region of human AMPH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAMPH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AMPH
Synonyms AMPH1
Function May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Cellular Location Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cytoskeleton
Tissue Location Neurons, certain endocrine cell types and spermatocytes
Research Areas
Citations (0)
citation

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Background

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.

References

He, P., et al. Amino Acids 38(4):1209-1218(2010)
Liu, L., et al. Protein Pept. Lett. 17(2):246-253(2010)
Hou, T., et al. J. Mol. Biol. 376(4):1201-1214(2008)
Zhou, P., et al. Biopolymers 90(6):792-802(2008)
Ballif, B.A., et al. Mol. Cell Proteomics 3(11):1093-1101(2004)

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$ 182.50
$ 70.00
Cat# AP19926C
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