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HBE1 Antibody(Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - HBE1 Antibody(Center) AP19854c
    All lanes : Anti-HBE1 Antibody (Center) at 1:8000 dilution Lane 1: human placenta lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • WB - HBE1 Antibody(Center) AP19854c
    All lanes : Anti- HBE1 Antibody(Center) at 1:1000 dilution Lane 1: human placenta lysate Lane 2: human kidney lysate Lane 3: human spleen lysate Lane 4: human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • WB - HBE1 Antibody(Center) AP19854c
    Anti- HBE1 Antibody(Center) at 1:1000 dilution + NCCIT whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 16 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P-Leica - HBE1 Antibody(Center) AP19854c
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using AP19854c performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
    detail
  • FC - HBE1 Antibody(Center) AP19854c
    Overlay histogram showing K562 cells stained with AP19854c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP19854c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, IHC-P-Leica, E
Primary Accession P02100
Other Accession P02103, NP_005321.1
Reactivity Human
Predicted Rabbit
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 16203 Da
Antigen Region 55-83 aa
Additional Information
Gene ID 3046
Other Names Hemoglobin subunit epsilon, Epsilon-globin, Hemoglobin epsilon chain, HBE1, HBE
Target/Specificity This HBE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human HBE1.
Dilution WB~~1:1000
IHC-P-Leica~~1:500
FC~~1:25
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHBE1 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HBE1
Synonyms HBE
Function The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.
Tissue Location Red blood cells.
Research Areas
Citations ( 0 )

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3'

References

Nuinoon, M., et al. Hum. Genet. (2009) In press :
Tachavanich, K., et al. Southeast Asian J. Trop. Med. Public Health 40(2):306-316(2009)
Naka, I., et al. Biochem. Genet. 46 (11-12), 708-711 (2008) :
Winichagoon, P., et al. Transl Res 152(4):178-184(2008)
Das, B., et al. Ann. Hum. Biol. 35(4):422-431(2008)

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$ 365.00
$ 140.00
Cat# AP19854c
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