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MBOAT7 Antibody(Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MBOAT7 Antibody(Center) AP19694c
    MBOAT7 Antibody (Center) (Cat. #AP19694c) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MBOAT7 antibody detected the MBOAT7 protein (arrow).
    detail
  • WB - MBOAT7 Antibody(Center) AP19694c
    Anti-MBOAT7 Antibody (Center) at 1:2000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 53 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q96N66
Other Accession Q8CHK3, Q0VCY6, NP_001139528.1, NP_001139554.1, NP_001139555.1, NP_077274.3
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52765 Da
Antigen Region 151-180 aa
Additional Information
Gene ID 79143
Other Names Lysophospholipid acyltransferase 7, LPLAT 7, 231-, 1-acylglycerophosphatidylinositol O-acyltransferase, 231n4, Bladder and breast carcinoma-overexpressed gene 1 protein, Leukocyte receptor cluster member 4, Lysophosphatidylinositol acyltransferase, LPIAT, Lyso-PI acyltransferase, Membrane-bound O-acyltransferase domain-containing protein 7, O-acyltransferase domain-containing protein 7, h-mboa-7, MBOAT7, BB1, LENG4, OACT7
Target/Specificity This MBOAT7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-180 amino acids from the Central region of human MBOAT7.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMBOAT7 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MBOAT7 (HGNC:15505)
Synonyms BB1, LENG4, OACT7
Function Acyltransferase which catalyzes the transfer of an acyl group from an acyl-CoA to a lysophosphatidylinositol (1- acylglycerophosphatidylinositol or LPI) leading to the production of a phosphatidylinositol (1,2-diacyl-sn-glycero-3-phosphoinositol or PI) and participates in the reacylation step of the phospholipid remodeling pathway also known as the Lands cycle (PubMed:18094042, PubMed:18772128). Prefers arachidonoyl-CoA as the acyl donor, thus contributing to the regulation of free levels arachidonic acid in cell (PubMed:18094042, PubMed:18772128). In liver, participates in the regulation of triglyceride metabolism through the phosphatidylinositol acyl-chain remodeling regulation (PubMed:32253259).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Localized in specific membrane structures termed mitochondria-associated membranes (MAMs) which connect the endoplasmic reticulum (ER) and the mitochondria.
Tissue Location Overexpressed in metastatic breast and bladder carcinomas relative to normal breast epithelium and urothelium
Research Areas
Citations (0)
citation

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Background

This gene encodes a member of the membrane-bound O-acyltransferases family of integral membrane proteins that have acyltransferase activity. The encoded protein is a lysophosphatidylinositol acyltransferase that has specificity for arachidonoyl-CoA as an acyl donor. This protein is involved in the reacylation of phospholipids as part of the phospholipid remodeling pathway known as the Land cycle. Alternative splicing results in multiple transcript variants.

References

Gijon, M.A., et al. J. Biol. Chem. 283(44):30235-30245(2008)
Lee, H.C., et al. Mol. Biol. Cell 19(3):1174-1184(2008)
Wende, H., et al. Immunogenetics 51 (8-9), 703-713 (2000) :
Fukunaga-Johnson, N., et al. Anticancer Res. 16 (3A), 1085-1090 (1996) :

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$ 365.00
$ 140.00
Cat# AP19694c
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