DPEP1 Antibody(N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | P16444 |
Other Accession | NP_001121613.1 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 45674 Da |
Antigen Region | 1-30 aa |
Gene ID | 1800 |
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Other Names | Dipeptidase 1, Dehydropeptidase-I, Microsomal dipeptidase, Renal dipeptidase, hRDP, DPEP1, MDP, RDP |
Target/Specificity | This DPEP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DPEP1. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | DPEP1 Antibody(N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | DPEP1 |
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Synonyms | MDP {ECO:0000303|PubMed:2303490}, RDP |
Function | Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4 (PubMed:2303490, PubMed:31442408, PubMed:32325220, PubMed:6334084). Hydrolyzes cystinyl- bis-glycine (cys-bis-gly) formed during glutathione degradation (PubMed:32325220). Possesses also beta lactamase activity and can hydrolyze the beta-lactam antibiotic imipenem (PubMed:32325220, PubMed:6334084). |
Cellular Location | Apical cell membrane; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI-anchor. Note=Brush border membrane {ECO:0000250|UniProtKB:P31429} |
Tissue Location | Expressed in lung and kidneys. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renal metabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982 [PubMed 6122685]). DPEP1 is responsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984 [PubMed 6334084]). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probable function was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibit selective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990 [PubMed 2303490]).
References
Nan, H., et al. J. Invest. Dermatol. 129(9):2250-2257(2009)
Pare, G., et al. Circ Cardiovasc Genet 2(2):142-150(2009)
Nitanai, Y., et al. J. Mol. Biol. 321(2):177-184(2002)
Kera, Y., et al. Comp. Biochem. Physiol. B, Biochem. Mol. Biol. 123(1):53-58(1999)
Satoh, S., et al. Biotechnol. Prog. 10(2):134-140(1994)
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