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ESR1 isoform4 Antibody(Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ESR1 isoform4 Antibody(Center) AP19656C
    Fluorescent confocal image of MCF-7 cell stained with ESR1 isoform4 Antibody (Center)(Cat#AP19656c). MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ESR1 isoform4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).ESR1 isoform4 immunoreactivity is localized to cytoplasm significantly.
    detail
  • WB - ESR1 isoform4 Antibody(Center) AP19656C
    ESR1 isoform4 Antibody (Center) (Cat. #AP19656c) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR1 isoform4 antibody detected the ESR1 isoform4 protein (arrow).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession P03372-4
Other Accession NP_000116.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35.5K KDa
Antigen Region 271-300 aa
Additional Information
Other Names ESR1; ESR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1
Target/Specificity This ESR1 isoform4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the C-term region of human ESR1 isoform4.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsESR1 isoform4 Antibody(Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)
citation

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Background

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

References

Geradts, J., et al. Cancer Invest. 28(9):969-977(2010)
Hayes, D.F., et al. Clin. Pharmacol. Ther. 88(5):626-629(2010)
Lupien, M., et al. Genes Dev. 24(19):2219-2227(2010)
Corbo, R.M., et al. J. Gerontol. A Biol. Sci. Med. Sci. (2010) In press :
Kim, S., et al. Fertil. Steril. (2010) In press :

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$ 365.00
$ 140.00
Cat# AP19656C
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