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Mouse Gpi Antibody(C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Gpi Antibody(C-term) AP19643b
    Mouse Gpi Antibody (C-term) (Cat. #AP19643b) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Mouse Gpi antibody detected the Mouse Gpi protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P06745
Other Accession NP_032181.2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 62767 Da
Antigen Region 532-558 aa
Additional Information
Gene ID 14751
Other Names Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, AMF, Neuroleukin, NLK, Phosphoglucose isomerase, PGI, Phosphohexose isomerase, PHI, Gpi, Gpi1
Target/Specificity This Mouse Gpi antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 532-558 amino acids from the C-terminal region of mouse Gpi.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Gpi Antibody(C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Gpi {ECO:0000303|PubMed:7545951}
Function In the cytoplasm, catalyzes the conversion of glucose-6- phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed:2344351, PubMed:7277315, PubMed:8417789). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (By similarity). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed:3352745, PubMed:3764429). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed:3352745).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:P06744}. Secreted {ECO:0000250|UniProtKB:P06744}
Research Areas
Citations (0)
citation

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Background

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

References

Benveniste, P., et al. Cell Stem Cell 6(1):48-58(2010)
Repiso, A., et al. Anat Histol Embryol 37(5):380-382(2008)
Szuber, N., et al. Exp. Hematol. 36(7):773-785(2008)
Chandele, A., et al. J. Immunol. 180(8):5309-5319(2008)
Cuda, C.M., et al. J. Immunol. 179(11):7439-7447(2007)

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$ 365.00
$ 140.00
Cat# AP19643b
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